Analysis Note

Negative Controltrp E (Ab-1)

Positive ControlMCF7, or A431 cells or breast carcinoma tissue

Application

Flow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)r>Frozen Sections (see application references)r>Immunoblotting (1 µg/ml)r>Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)r>Immunoprecipitation (see application references)r>Neutralization Studies (see application references)r>Paraffin Sections (5 µg/ml, heat pre-treatment required)

General description

This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.

Recognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.

Immunogen

recombinant human cyclin D1

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.

Inaba, T., et al. 1992. Genomics13, 565.Jiang, W., et al. 1992. Cancer Res.2, 2980.Pines, J. 1992. Cell Growth Differ.2, 305.Xiong, Y., et al. 1992. Genomics13, 575.Xiong, Y., et al. 1992. Cell71, 504.Motokura, T., et al. 1991. Nature350, 512.Solomon, M.J., et al. 1990. Cell63, 1013.Draetta, G., and Beach, D. 1988. Cell54, 17.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Warning

Toxicity: Standard Handling (A)